Inhibition of pseudotype virus entry by existing FDA-approved drugs

293T cells were transfected 24hours previous then mixed with set dilutions of drug and infected with PVs (carried out in triplicate). After 48hours incubation firefly and Renilla values were measured using FLUOstar Omega plate reader (BMG Labtech). Firelfly values were normalised to the corresponding Renilla value. Percent infection at each dilution was calculated using the average values of the untreated control. *values for both ESOM and OM were shared and performed on the same 96-well plate.

在实验中，对293T细胞进行了24小时的转染处理，随后将其与一系列药物稀释液混合，并感染了PVs（该步骤重复三次）。经过48小时的培养后，利用FLUOstar Omega板式阅读仪（BMG Labtech生产）测量了萤火虫荧光素酶和Renilla荧光素酶的值。萤火虫荧光素酶的值被标准化为相应的Renilla荧光素酶值。在每个稀释度下的感染百分比是通过未处理对照组的平均值计算得出的。ESOM和OM的值均共享，并在同一96孔板上进行测量。