Supplemental Tables 1-8 from Colorectal Cancer Metastases in the Liver Establish Immunosuppressive Spatial Networking between Tumor-Associated SPP1+ Macrophages and Fibroblasts

Supplemental Table 1: Antibodies, reporters, imaging order and exposure times used in CODEX imaging. EPCAM, SPP1, CD163 were excluded from downstream analysis due to non-specific staining; Supplemental Table 2: Sequencing metrics for scRNA-seq; Supplemental Table 3: Absolute number of cells detected from each sample per cell lineage; Supplemental Table 4: Differentially expressed genes belonging to respective macrophage clusters compared by site of sample origin. pct.1, pct.2 indicate percentage of cells expressing given gene in the cluster of interest and all other comparison clusters respectively; Supplemental Table 5: Enriched reactome pathways in differentially expressed genes in tumor macrophages; Supplemental Table 6: Gene signatures for Foam cells (Fernandez et.al., 2020, PMID 31591603) and scar-associated macrophages (Ramachandran et. al., 2020, PMID 31597160); Supplemental Table 7: Annotation of differentially expressed genes in CAFs with the components of the matrisome expression program; Supplemental Table 8: Spatial proximity analysis examining whether fibroblasts are more proximal to macrophages than any other cell type.