Sequence alignment of L. donovani aquaporins at the first and second NPA box.

The sequences around the two NPA (underlined) are aligned. NPAs (asparagines-proline-alanine) are underlined. The second NPA box of L. donovani AQPs with the exception of AQP1 are similar to tonoplast integral protein (TIP1.1) which is localized to intracellular organelles. Small basic intrinsic proteins (SIP1.1) are plant subcellular aquaporins. Tip1.1 sequence is from a plant Arabidopsis thaliana (Q9ZV07). SIP1.1 sequence is also from Arabidopsis thaliana (Q9M8W5). The downstream sequence of the second NPA box of AQP1 has arginine (R) and is replaced by valine (V) or alanine (A) in the remaining LdAQPs.