LC–MS/MS Proteomic Profiling of PEAK3 S69 Phosphorylation in PEAK3 Mutants

This project aims to investigate how different PEAK3 mutations affect phosphorylation at serine 69 (S69). To this end, we performed proteomic analyses on immunoprecipitated (IP) PEAK3 samples from both wild-type and mutant constructs. Peptides containing phosphorylated and non-phosphorylated S69 were specifically identified and compared to assess mutation-dependent changes in S69 phosphorylation.