Cyclosporin A inhibits prostate cancer growth through suppression of E2F8

Cyclosporin A (CsA) is a potent immunosuppressive agent used clinically in organ transplantation. In this study, we examined that CsA exerts anti-tumor activity against prostate cancer. We performed microarray experiments to investigate the effect of CsA on the transcriptome of prostate cancer cells. CsA potently induced transcriptome change and primarily affected cell cycle-related gene signatures. These results suggest that CsA can be used as a potential therapeutic agent or an intriguing tool for exploring prostate cancer biology and identifying novel therapeutic targets. Cells were cultured in RPMI1640 medium containing 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 ug/ml streptomycin. CsA was dissolved in 100% ethanol (vehicle) and treated to cells with 10 mM CsA for 24h