scRNA-Seq and snRNA-Seq in human adipose tissue

From subcutaneous adipsoe tissue from 2 particpants we performed scRNA-seq on the stromal vascular fraction, snRNA-seq on isolated adipocytes and snRNA-seq on frozen adipose tissue. We performed clustering analysis, differential gene expression analysis and over-representation analysis to decipher the different cell populations detectable with each technique. The SVF fraction was able to show more diverse immune cell populations. Whilst the snRNA-seq frozen adipose tissue showed diverse clusters of adipocytes that was not observable from a snRNA-Seq fraction Subcutaneous adipose tissue from two participants subjected to scRNA-seq on the stromal vascular fraction, snRNA-seq on isolated adipocytes and snRNA-seq on frozen adipose tissue