Ptbp1 deletion does not induce glia-to-neuron conversion in adult mouse retina and brain.

Purpose: scRNA-Seq was performed to profile changes in adult retina Muller glia cells and brain astrocytes after Ptbp1 deletion Overall design: Tissues (retina, cerebral cortices, striatum, and midbrain) were dissected into Hibernate-A media with a 2% B-27 and GlutaMAX supplement (0.5 mM final). Tissues were dissociated in papain (Worthington). Cells were then loaded into 10x Genomics Chromium Single Cell System (10x Genomics) and libraries were generated using v3.1 chemistry following the manufacturer's instructions. Libraries were sequenced on Illumina NovaSeq. Sequencing data were first processed through the Cell Ranger (v.6.0.1, 10x Genomics) with default parameters, aligned to the mm10 genome (refdata-gex-mm10-2020-A), and matrix files were used for subsequent bioinformatic analysis.