Figure S1 - HRES-1/Rab4 Promotes the Formation of LC3+ Autophagosomes and the Accumulation of Mitochondria during Autophagy

Flow cytometry detection of HRES-1/Rab4 isoforms, including wild-type HRES-1/Rab4, C-terminally truncated HRES-1/Rab41–121, dominant-negative/GTP binding-deficient HRES-1/Rab4S27N, constitutively active/GTPase-deficient HRES-1/Rab4Q72L and phosphorylation-resistant form HRES-1/Rab4S204Q, tagged with eGFP and LC3 fused to FP650 (FP650-LC3). A) Flow cytometry of HeLa cells transfected with backbone vector pAAV-MCS and expression constructs producing HRES-1/Rab4-eGFP fusion proteins and eGFP alone. B, Flow cytometry of HeLa cells transfected with backbone vector pAAV-MCS, and expression vectors producing FP650-LC3 fusion protein and FP650 alone. (PDF)