Transcriptomic analysis of hypersegmented human neutrophils

We report the characterisation of the transcriptional changes associated with neutrophil hypersegmentation in primary human cells. We established a model of hypersegmentation by exposing healthy peripheral blood neutrophils to the angiotensin converting enzyme inhibitor (ACEi) captopril. Laser capture microdissection (LCM) was then adapted to isolate a population of hypersegmented neutrophils. Transcriptomic analysis of microdissected hypersegmented neutrophils was undertaken using RNA sequencing. This study reveals the transcriptomic signature of hypersegmented neutrophils, with five genes differentially expressed and modulated pathways including histone modification, protein-DNA complex assembly and antimicrobial humoral response. Overall design: Transcriptomic analysis of hypersegmented human neutrophils.