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Effects of high cholesterol diet on sperm small RNA expression profile utilizing two different RNA-seq methods

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP479314
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Purpose: To reveal the small RNA profiles in the sperm using traitional and PANDORA-seq methods Methods: 3 week old LDLR-/- male mice were fed a high cholesterol or low cholesterol diet for 9 weeks. Sperm RNA was extracted via Trizol. Results: paternal HCD feeding can lead to sperm tsRNA/rsRNA landscape changes that are undetected by traditional RNA-seq Overall design: Sperm small RNA were sequenced from LDLR-/- male mice after 9 weeks of low cholesterol or high cholesterol feeding
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2024-10-09
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