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RNAseq analysis of the serotype M1 group A Streptococcus strain MGAS2221. Streptococcus pyogenes

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NIAID Data Ecosystem2026-03-07 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA193454
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We recovered RNA from exponential and stationary phase cell cultures of MGAS2221 and utilized two distinct methods to remove rRNA from our samples, the RiboZero kit from Epicenter, and the terminator- 5--Phosphate-Dependent Exonuclease (TEX). This gave us 4 distinct RNAs with which we performed RNAseq analysis. Both rRNA removal methods worked well, with only a small percentage of the final total read counts being derived from rRNA sequences. Overall design: A single group A Streptococcus strain was investigated.
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2013-03-19
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