George von Dassow, Koen J.C. Verbrugghe, Ann L. Miller, Jenny R. Sider, William M. Bement (2011) CIL:15793, Strongylocentrotus purpuratus. CIL. Dataset

16-cell purple urchin embryo with EMTB-3G (microtubules) in shown cyan and mC-H2B (chromatin) in yellow. The embryo is treated with 15 µM trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells at time 00:00. Because of a slight asynchrony in mitosis at the time of TSA addition, each macromere exhibits a distinct phenotype. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C. The video corresponds to the second segment of video 5 and Fig. S3B in J Cell Biol. 2009 Dec 14;187(6):831-45. Note the control untreated video is the is also part of this image group.

在本次研究中，展示了16细胞紫海胆胚胎中的微管（EMTB-3G）以青色呈现，以及组蛋白H2B（染色质）以黄色显示。该胚胎经15 µM三环素A（TSA）处理，TSA是一种HDAC6（微管去乙酰化酶）抑制剂，可选择性破坏哺乳细胞中动态微管的稳定性，处理时间为00:00。由于在TSA添加时存在轻微的细胞分裂异步性，每个大单体表现出独特的表型。胚胎图像是通过安装在复合显微镜（尼康E800）上的激光扫描共聚焦显微镜（Bio-Rad实验室的Radiance 2000）使用40× 1.3 NA Plan-Fluor油镜头进行成像。室温保持在14–17°C。该视频对应于J Cell Biol. 2009年12月14日第187卷第6期的视频5的第二部分以及图S3B。请注意，未经处理的对照组视频也是本图像组的一部分。