Table_1_Monthly dynamics of microbial communities and variation of nitrogen-cycling genes in an industrial-scale expanded granular sludge bed reactor.XLSX

IntroductionThe expanded granular sludge bed (EGSB) is a major form of anaerobic digestion system during wastewater treatment. Yet, the dynamics of microbial and viral communities and members functioning in nitrogen cycling along with monthly changing physicochemical properties have not been well elucidated.MethodsHere, by collecting the anaerobic activated sludge samples from a continuously operating industrial-scale EGSB reactor, we conducted 16S rRNA gene amplicon sequencing and metagenome sequencing to reveal the microbial community structure and variation with the ever-changing physicochemical properties along within a year.ResultsWe observed a clear monthly variation of microbial community structures, while COD, the ratio of volatile suspended solids (VSS) to total suspended solids (TSS) (VSS/TSS ratio), and temperature were predominant factors in shaping community dissimilarities examined by generalized boosted regression modeling (GBM) analysis. Meanwhile, a significant correlation was found between the changing physicochemical properties and microbial communities (p

引言：扩展精细污泥床（EGSB）是废水处理过程中主要的厌氧消化系统形式。然而，微生物和病毒群落及其在氮循环中的功能，以及随月份变化的物理化学性质的动力机制尚未得到充分阐明。方法：在本研究中，我们从连续运行的大型工业规模EGSB反应器中收集厌氧活性污泥样品，通过16S rRNA基因扩增子测序和宏基因组测序，揭示了微生物群落结构及其在一年内随物理化学性质变化而发生的变异。结果：我们观察到微生物群落结构呈现明显的月度变化，化学需氧量（COD）、挥发性悬浮固体（VSS）与总悬浮固体（TSS）之比（VSS/TSS比）以及温度是塑造所考察群落差异的主要因素，这一发现是通过广义提升回归模型（GBM）分析得出的。同时，发现物理化学性质的变化与微生物群落之间存在显著的相关性（p