利用全细胞催化结合电渗析分离的生物过程智能去除低聚木糖液中杂质

Acetic acid hydrolysis is a green and novel approach that can effectively convert lignocellulosic biomass into high-valued xylo-oligosaccharide (XOS). However, the residual acidolysis slurry still contains acetic acid and various monosaccharides. In this study, a sandwich-integration bioprocess (SIBP) method was developed to efficiently remove the residual monosaccharide contaminants by the combination of whole-cell catalysis and electrodialysis separation. Firstly, the acetic acid in acidolysis slurry was separated by electrodialysis to eliminate the strong toxic inhibition on whole-cell catalysis. Secondly, the monosaccharides were oxidized to the corresponding ionogenic acids by whole-cell catalysis. Finally, these ionogenic aldonic acids were removed readily by the second electrodialysis step.