CIL:25824

Chromophore-assisted laser inactivation (CALI) inactivates target proteins by generating reactive photoproducts such as reactive oxygen species (ROS) by intense irradiation of photosensitizer chromophores, such as EGFP. Rat2 cells expressing soluble EGFP, were subjected to CALI to test for nonspecific laser-induced effects. After administration of a 100-ms dose of 1.5 mW/[μm]2 laser light, no morphological changes were visualized, as as seen in this DIC time-lapse movie. This and CIL# 25823 are control movies for CIL# 25821, 25822. This movie is Movie4 in Supporting Information in Proc Natl Acad Sci (2007) 104: 6702-6707.