Supplementary information of Novel therapeutic strategy for intractable keloids: Suppression of intracellular mechanotransduction and actin polymerization via Rho-kinase pathway inhibition

Supplementary materials and supplementary figure legends.
Supplementary figure S1. Expression of ROCK1 in keloid tissues and fibroblasts.
Supplementary figure S2. Mechanical stress induces ROCK1 expression and F-actin rearrangement in keloid fibroblasts.
Supplementary figure S3. Suppressed ROCK1 induces F-actin rearrangement in KFs through Rho/ROCK1 transduction pathway.
Supplementary figure S4. Effect of Y27632 on cytoskeletal rearrangement of F-actin in KFs.
Supplementary figure S5. Inhibition of ROCK1 attenuates skin fibrosis and cell migration in KFs.
Supplementary figure S6. Cyclic stretch-activated ROCK1 triggers nuclear translocation of YAP and MRTF.
Supplementary figure S7. Histological and immunohistochemical images of nodules formed by human KFs in SCID mice 7 days post-Y27632 treatment.
Supplementary figure S8. Histological images of nodules formed by human KFs in SCID mice 7 days post-Y27632 treatment.
Supplementary figure S9. Immunohistochemical images of the nodules formed by human KFs in SCID mice 7 days post Y27632 treatment.