Exacerbated reduction of OPA1 and MFN2 in ischemic 5xFAD mice.

(A) The expression levels of long OPA1 isoform (OPA1-L) and short OPA1 isoform (OPA1-S) were substantially reduced in brain mitochondria from ischemic 5xFAD mice; while the ratio of OPA1-L to OPA1-S remained unchanged. *P<0.05 vs other groups. (B) The expression level of MFN2 was significantly reduced in brain mitochondria from ischemic 5xFAD mice. *P<0.05 vs other groups. The levels of Dlp-1 translocation to mitochondria (C) or the ratios of phosphor-Dlp1 to Dlp1 in brain homogenate (D) in the four groups of mice were not changed. *P<0.05 vs other groups. (E) Representative immunoreactive bands of OPA1-L, OPA1-S and MFN2 in mitochondrial fractions. (F) Representative immunoreactive bands of Dlp-1 in mitochondrial fractions. (G) Representative immunoreactive bands of Dlp-1 and phospho-Dlp-1 in brain homogenates. Mitochondrial matrix protein Hsp60 and outer mitochondrial membrane protein VDAC were used to show the enrichment of mitochondrial fraction as well as the equal loading amount of the samples. β-actin was used to show the equal loading amount of tissue samples. N = 4–6 mice of each group.