scRNA-SEQ analysis of SW620 xenografts tumor in nude mice

In order to illustrate the change of SW620 xenografts in nude mice, we collected SW620 xenografts at Week5 after the tumor cells were injected subcutaneously in nude mouse. Tumor tissues were collected and disocciated to single cells and subjected to scRNA-SEQ. Overall design: There were four mice with subcutaneous tumor. All of these mice were injected with SW620 subcutaneously and fed for 5 weeks. Then mice were sacrificed and tumor tissues were collected and subject to single cell dissociation. Live cells were enriched and subjected to scRNA-SEQ analysis.