Miguel Vicente-Manzanares, Margaret A. Koach, Leanna Whitmore, Marcelo L. Lamers (2011) CIL:12411, Cricetulus griseus, epithelial cell. CIL. Dataset

Localization and intracellular motility of myosin IIB (MIIB) in protrusive regions of migrating CHO.K1 cells. This Video corresponds to Fig. 6 B and video 4 in JCB 183:543-554 , 2008. Images of GFP-MIIB (green) and RFP-actin (magenta) were collected every 4 s using TIRF microscopy with 10 frames/s shown. Images were acquired in an inverted microscope (model IX70;Olympus). GFP and RFP were excited using the 488-nm laser line of an Ar ion laser and the 543-nm laser line of a He-Ne laser (Melles Griot), respectively. For dual GFP- mRFP/mCherry/mOrange, a dual-emission filter (z488/543) was used. Images were acquired with a charge-coupled device camera (Retiga Exi; Qimaging) and analyzed using MetaMorph software (MDS Analytical Technologies).

关于迁移CHO.K1细胞突起区域肌球蛋白IIB（MIIB）的定位和细胞内运动的研究。本视频对应于JCB 183:543-554 (2008) 文章中的图6B和视频4。使用TIRF显微镜，以每秒10帧的速度，每隔4秒采集了GFP-MIIB（绿色）和RFP-肌动蛋白（品红色）的图像。图像采集于倒置显微镜（型号IX70；Olympus）进行。GFP和RFP分别由488纳米的Ar离子激光和543纳米的He-Ne激光（Melles Griot）激发。对于双重发射的GFP-mRFP/mCherry/mOrange，使用了双发射滤光片（z488/543）。图像通过电荷耦合器件相机（Retiga Exi；Qimaging）采集，并利用MetaMorph软件（MDS Analytical Technologies）进行分析。