Transcription profiling of ALK-rearranged cell lines resistent and sensitive to crizotinib

In this study we have analysed the transcription profile of ALK rearranged cell lines (COST, DEL, JB6, KARPAS, SR786, SUPM2, TS). The analysis was done on untreated cell lines and on the te same cell lines surviving to progressive increasing addition of crizotinib to the culture medium. Crizotinib concentration was progressively increased to reach a final concentration of 1 μM. The resulting pool of resistant cells (designated crizotinib-resistant (CR)) were maintained in RPMI with 10% FBS containing 0.5-1 μM crizotinib. Seven ALK rearranged cell lines, which became resistent to crizotinib, were compared at transcription level with the corresponding untreated cell lines.