PRO-seq assay in adult ventricular cardiomyocytes

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE134800

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PRO-seq assay was performed for the quantitative detection of nascent pre-mRNA transcripts induced by phenylephrine (PE) and adenoviral overexpression of SRF serine-103 mutants in adult ventricular cardiomyocytes Adult ventricular cardiomyocytes were stimulated with PE 24 hours after adenoviral infection with SRF WT, S103A and S103D mutants. Nuclei were collected 1 hour after PE treatment, then subjected to precision nuclear run-on sequencing (PRO-seq).

创建时间：

2020-12-09