Jennifer Bestman, Holly Cline (2011) CIL:36911, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This IMAGE #3 (day 3)of a time lapse series contained with in this image group. It shows a neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.

The images were acquired daily over 3 days and show dendritic arbor development and morphological plasticity of the dendritic arbor with branch additions and retractions.

This cell was transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

本图像组中包含的延时摄影系列图像#3（第3天）所示。该图像呈现了无色斑马鱼Xenopus laevis蝌蚪中枢神经系统视顶盖中神经元的发展过程，处于第47个发育阶段。图像是在连续3天每日采集得到的，展示了树突丛的发展及其形态的可塑性变化，包括分支的增加和退缩。该细胞在首次图像采集前约24小时通过原位电穿孔转染编码eYFP的质粒DNA。完整的活体蝌蚪被麻醉，置于玻璃盖玻片下，并通过定制构建的双光子显微镜直接穿过其透明皮肤进行拍摄。