Day-night rhythm in murine retinal pigment epithelium

Murine RPE transcriptomes were generated for 6 time points during a 24 hour period. Our data demonstrates that genes involved in ATP generation have a high expression at night whereas genes involved in β-oxidation and cholesterol synthesis show a high expression in the morning. Additionally, genes were identified in the glycerophospholipid metabolism pathway, suggesting the generation of glycerophospholipids at night. Finally, genes involved in intracellular trafficking with different expression patterns during the day-night cycle were identified and we identified genes involved in actin cytoskeleton building, remodelling and crosslinking with a high expression in the morning. Murine RPE transcriptomes were generated for 6 time points during a 24 hour period (ZT0, 2, 4, 9, 14, 19). In total 30 samples were sequenced because each time point was repeated 5 times (n=5).本研究获取了24小时周期内6个时间点的小鼠视网膜色素上皮（Murine RPE）转录组测序数据。本研究数据显示，参与ATP生成的基因在夜间呈现高表达特征，而参与β氧化（β-oxidation）与胆固醇合成的基因则于清晨达到高表达水平。此外，本研究在甘油磷脂代谢通路（glycerophospholipid metabolism pathway）中鉴定到一批相关基因，提示夜间存在甘油磷脂的合成过程。最后，本研究鉴定到一批在昼夜周期中呈现差异化表达模式的细胞内转运相关基因；同时还鉴定到参与肌动蛋白细胞骨架构建、重塑与交联的基因，此类基因于清晨呈现高表达水平。本研究同样针对24小时周期内的6个时间点完成了小鼠视网膜色素上皮转录组测序，具体时间点为授时因子时间（Zeitgeber Time, ZT）0、2、4、9、14及19。由于每个时间点设置5次生物学重复（n=5），最终共完成30例样本的测序。