Genome-wide maps of chromatin state (heterochromatin H3K9me2 or centromeric histone H3 variant CENP-A/Cnp1) in both mhf2? and mhf2+ carrying the inactivated Centromere 1 or Centromere 2.

We report the high-throughput profiling of histone modification (H3K9me2) or histone variant CNEP-A/Cnp1 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide H3K9me2 or CENP-A/Cnp1 maps of both mhf2? and mhf2+ carrying the inactivated Centromere 1 or Centromere 2 in fission yeast. We find that neocemtromeres are formed preferably at pericentromeric regions in single depletion of CENP-T-W-X-S. Overall design: 4 mhf2? and mhf2+ carrying the inactivated Centromere 1 or Centromere 2 were examined by anti-CENP-A/Cnp1 ChIP-seq. 2 mhf2+ carrying the inactivated Centromere 1 or Centromere 2 were examined by anti-H3K9me2 ChIP-seq.