MBX-4132 Mycobacterium tuberculosis

RNA-seq was performed on Mycobacterium tuberculosis H37Rv cultures treated with 1.2 uM of MBX-4132 or equal-volume DMSO, for 48 hours in high-zinc MtbMinimal Media. In addition, RNA-seq was also carried out on M. tuberculosis H37Rv CRISPRi nontargeting control and ssr knockdown strains. Both strains were induced in high-zinc MtbMinimal Media for 7 days. Transposon insertion sequencing was performed on a saturated library in Mycobacterium tuberculosis H37Rv, where cells were treated with 0.5 uM MBX-4132 or equal-volume DMSO for ~5 generations. Whole-genome resequencing was carried out on the H37Rv C- (labeled as altRP in the file name) knockout strain to verify successful deletion of the operon.