Effects of sucrose and artificial sweeteners on glucose and lipid metabolism in mice

Excessive sugar intake is increasingly recognized as a cause of obesity and related metabolic diseases. To reduce sugar intake, artificial sweeteners, which are sweet and very low in calories, are widely used in many foods and beverages. Sucralose is currently the most commonly used low-calorie sweetener, but the scientific community has not yet reached a consensus on the safety of sucralose, especially its impact on glucose metabolism. Effects of isosweet sucrose on glucose and lipid metabolism in mice.The RNA from the liver was isolated via the TRIzol reagent. The RNA sequencing library library preparation was subjected to sequencing via an Illumina NovaSeq 6000 platform by CapitalBio Technology (PRC). Fold-change screening was used to find genes that were differentially expressed. GO Enrichments was studied in a command-line software KOBAS 2.0. To statistically identify the highly enriched pathways in a batch of sequences, our team utilized the complete genome as the default background distribution. For every pathway occurring in the set of genes, our team recorded the sum of genes in the set involved in the pathway term or the GO term. A hypergeometry distribution was used to determine the p value.