Richard Allen (University of Hawaii), D. Kunkel (2011) CIL:36285, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

A thin cryosection of Vorticella exposed to a rabbit polyclonal antibody to alpha-centrin and then to gold conjugated to anti-rabbit IgG. The gold indicates that the myoneme contains alpha-centrin. The plasma membrane, outer alveolar membrane and inner alveolar membrane all appear as transparent tracks but the lumen of the alveolus appears electron opaque. Thickenings of the ER lying against the myoneme correspond to linkage complexes. TEM taken on 8/18/94 by D. Kunkel with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm.

一薄片状的涡虫，经兔多克隆抗 alpha-centrin 抗体染色，随后用与抗兔 IgG 结合的金进行标记。金标记表明肌动蛋白包含 alpha-centrin。质膜、外肺泡膜和内肺泡膜均呈现为透明的轨迹，但肺泡的腔室则呈现电子不透明。位于肌动蛋白附近的粗面内质网增厚与连接复合体相对应。1984年8月18日由D. Kunkel使用80kV运行的蔡司10A透射电子显微镜（TEM）拍摄。负片放大倍数为19,800倍。标尺长度为0.2微米。