CELeidoscope (ZAM47) spectral flow cytometry data and FlowJo workspace

Flow cytometry data and analysis files from CELeidoscope (ZAM47) Caenorhabditis elegans single-cell suspensions. This dataset includes raw flow cytometry standard (FCS) files, CellView workspace (CVW) files, and FlowJo workspace files (.wsp) used for spectral unmixing, gating, and population identification. The FlowJo workspace dated 2025-03-07 was used to define single-color controls for spectral unmixing. Associated FCS files include: ZAM36 (YFP), ZAM37 (mCherry), ZAM39 (GFP), ZAM45 (mKO2), and ZAM47 (500k events). The FlowJo workspace dated 2025-08-15 corresponds to the final sorting and downstream transcriptomic analyses. Associated files include ZAM47 and ZAM47_2, along with their respective FCS and CVW files. Flow cytometry was performed on a BD FACSDiscover™ S8 spectral cell sorter. Data were analyzed using FlowJo v10.10 (BD Life Sciences) with the CellView Lens plugin for visualization of imaging-enabled cytometry data. These files support the analyses presented in CELeidoscope: a quad-fluorescent Caenorhabditis elegans strain for tissue-specific spectral single-cell analyses and enable reproduction of spectral unmixing, gating strategies, and population identification described in the study.