The exploration of cooperative bindings of two transcription factors by ChIP-seq of Atf1-Rst2 binding during glucose starvation in Schizosaccharomyces pombe.. Schizosaccharomyces pombe

Transcriptional regulation is pivotal biological process to adapt to environmental fluctuations and is achieved by the binding of numerous transcriptional factors (TFs) to their target sequences. TFs determine which genes to activate through binding to their binding sequences consisting of specific motif. However, how TFs choose appropriate targets from the numerous candidates in huge genome has not been fully elucidated. We found that the bindings of TFs to the binding sequences placed in close proximity cooperatively stabilize their binding, and thereby integrating distinct signal-pathways in fission yeast fbp1 gene. This study aims to identify cooperative two TFs binding sites. To this end, we co-precipitated genome DNA to where both TFs, Atf1 and Rst2, bind by the sequential-ChIP analysis using anti-Flag and anti-Atf1 antibodies in rst2-3flag cells, and analyzed the resultant ChIP DNA samples with next generation sequencer.