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Sequence information, specificity, amplicon size and molarity of oligonucleotide primers and probes used in the qPCR assays.

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Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Sequence_information_specificity_amplicon_size_and_molarity_of_oligonucleotide_primers_and_probes_used_in_the_qPCR_assays_/1107154
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a)F, forward primer; R, reverse primer; P, probe; FAM, 6-carboxyfluorescein; BHQ1, Black Hole Quencher 1.b)Published oligonucleotide sequences showing mismatches were modified by using the nucleotides which are underlined at these positions.c)In their study, Morillo and colleagues [28] designated the forward primer as reverse and the other way around. Here, the correct designation is given.
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2015-12-02
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