SAPCD2 promotes neuroblastoma progression by altering the subcellular distribution of E2F7

We report a previously unrecognized role of SAPCD2 in E2F signaling pathway in neuroblastoma. To explore SAPCD2-dependent gene regulation, RNA-seq analysis was conducted and the differently expressed genes were revealed in the SAPCD2-knockdown SK-N-BE(2) cells in comparison to control group. We find that SAPCD2 contributes to E2F7 mislocalization and decreases the level of nuclear E2F7 in NB cells, thus restraining the inhibitory effect of nuclear E2F7 on E2F signaling pathway. SAPCD2 knockdown limits the overactivation of E2F signaling in NB cells, affects the expression of genes involved in cell cycle and chromosome stability, and thereby blocking tumor progression. Overall design: mRNA profiles of SK-N-BE(2) cells transfected with scramble shRNA (sh-Scb) or sh-SAPCD2 were generated by RNA sequencing, in double, using Illumina NovaSeq6000.