Figure S1 - Monoclonal Antibodies to the V2 Domain of MN-rgp120: Fine Mapping of Epitopes and Inhibition of α4β7 Binding

Inhibition of rgp120 binding to CHO cell lines expressing CD4 by monoclonal antibodies to the V2 domain of gp120. CHO cells expressing membrane-bound human CD4 were plated in 96-well microtiter plates until they were 90% confluent. Serial dilutions of MAbs were pre-incubated with a fixed amount of [125I]-gp120 (2 nM) and then incubated with the cells for 2 hr. The cells were then washed four times and the cells solubilized in 100 µL of 0.1 N NaOH. Specific binding was calculated as described previously [27]. MAb 1024, a CD4-blocking antibody directed to the C4 domain of gp120, served as a positive control. (PDF)