The raw data of the anti-inflammatory role of zDHHC23 manuscript submitted to Frontiers in Immunology

Zinc finger Asp-His-His-Cys motif-containing (zDHHC) proteins, known for their palmitoyltransferase (PAT) activity, play crucial roles in diverse cellular processes, including immune regulation. However, their non-palmitoyltransferase immunomodulatory functions and involvement in teleost immune responses remain underexplored. In this study, we systematically characterized the zDHHC family in the large yellow croaker (<em>Larimichthys crocea</em>), identifying 22 members. Phylogenetic analysis unveiled that each of the 22 <em>Lc</em>zDHHCs formed distinct clusters with their orthologues from other teleost species. Furthermore, all <em>Lc</em>zDHHCs exhibited a highly conserved DHHC domain, as confirmed by tertiary structure prediction. Notably, <em>Lc</em>zDHHC23 exhibited the most pronounced upregulation following <em>Pseudomonas plecoglossicida</em> (<em>P. plecoglossicida</em>) infection of macrophage/monocyte cells (MO/MΦ). Silencing <em>Lc</em>zDHHC23 led to heightened pro-inflammatory cytokine expression and diminished anti-inflammatory cytokine levels in MO/MΦ during infection, indicating its anti-inflammatory role. Functionally, <em>Lc</em>zDHHC23 facilitated M2-type macrophage polarization, as evidenced by a significant skewing of MO/MΦ towards the pro-inflammatory M1 phenotype upon <em>Lc</em>zDHHC23 knockdown, along with the inhibition of MO/MΦ necroptosis induced by <em>P. plecoglossicida</em> infection. These findings highlight the non-PAT immunomodulatory function of <em>Lc</em>zDHHC23 in teleost immune regulation, broadening our understanding of zDHHC proteins in host-pathogen interactions, suggesting <em>Lc</em>zDHHC23 as a potential therapeutic target for immune modulation in aquatic species.