An SDS-PAGE electrophoretic separation of proteins from an crude whole cell lysate before () and after () purification in the PPC microchip

<b>Copyright information:</b>Taken from "Purification and preconcentration of genomic DNA from whole cell lysates using photoactivated polycarbonate (PPC) microfluidic chips"Nucleic Acids Research 2006;34(10):e74-e74.Published online 6 Jun 2006PMCID:PMC1475748.© The Author 2006. Published by Oxford University Press. All rights reserved Following thermal lysis, the cellular suspension was centrifuged at 3000 r.p.m. (735 × ) for 10 min. The supernatant (2 × 10 µl samples) was removed from the centrifuge tube and the remaining debris was rigorously vortexed and another two 10 µl samples were removed. The lanes in this figure represent standard sizing ladder (a); thermally lysed cells, supernatant (b and c); and thermally lysed cells, debris (d and e). Proteins were separated using a 7.5% acrylamide gel at 12 V/cm in 1× Tris–Glycine–SDS buffer, pH 8.8. Proteins were indexed against a sizing ladder, which ranged from 37 to 250 kDa in size.