Nip and ctb3 mutant RNA-seq under CS-PT treatment for 1 day

To identify the downstream genes regulated by CTB3, we collected panicles from the CTB3-cr1 and Nip plants after 1 day of CS-PT treatment and performed RNA sequencing (RNA-seq). We identified 1020 differentially expressed genes (DEGs), including 810 downregulated genes and 210 upregulated genes. GO enrichment analysis of DEGs identified several highly significant terms in biological processes and in molecular functions. We focused on GO terms related to energy supply and carbohydrate (sugar) metabolism. RNA-seq profiling of panicles from wildtype Nip (Nip) and CTB3 knockdown mutants (CTB3-cr1) at 1day of CS-PT cold treatment.