Experiment2

Data for experiment 2. To measure genetic conflict between attracting rhizobia and repelling nematodes, and to test how parasitic nematodes impact the rhizobia mutualism, we used a split-plot randomized design. Each block contained two treatments: one in which we only inoculated plants with rhizobia, and one in which we inoculated plants with both rhizobia and nematodes. Plants received a total of 400 nematode eggs from a genetically variable nematode inoculum. Each treatment in each block contained one M. truncatula individual from each of 50 genotypes. We replicated this design across 10 blocks (50 plants per treatment per block × 2 treatments × 10 blocks = 1000 plants).