Data Repository for Béchade et al. (2025). Species-specific bacterial associations emerge from stochastically assembled microbiomes in Northeastern American fireflies.

SHARING/ACCESS INFORMATION
Recommended citation for this repository: cite directly the published scientific article when available (Béchade et al. (2025), Species-specific bacterial associations emerge from stochastically assembled microbiomes in Northeastern American fireflies. Journal.).

DATA & FILE OVERVIEW (14 datasets)

REPOSITORY. Script for Bechade et al. firefly microbiome article.R: Complete R script used to process and analyze qPCR and sequencing data.REPOSITORY. Input data for R.rar: Input and intermediate files for data analysis in R.REPOSITORY. firefly_non-rarefied_otutable.csv: OTU table from phyloseq sequencing data analysis, including non-rarefied counts.REPOSITORY. firefly_rarefied_otutable.csv: OTU table from phyloseq sequencing data analysis, including rarefied counts at 30,000 reads.REPOSITORY. Rarefaction curves.pdf: Rarefaction curves.REPOSITORY. firefly16S_taxonomy_with_seqs.xlsx: Taxonomy table from phyloseq sequencing data analysis, including taxonomic classification of all sequenced variants and fasta sequences.REPOSITORY. contaminants.decontam.xlsx: Taxonomy table from phyloseq sequencing data analysis for variants classified as contaminant by our decontam run, including taxonomic classification of all sequenced reads and fasta sequences.REPOSITORY. Contaminant analysis 2.pdf: Examples of variants classified as contaminants or not based on the decontam frequency method.REPOSITORY. Percent reads retained, sequence length, and and distribution of sequencing depth 2.pdf: Information on the proportion of reads retained through quality filtering, length of sequences, and read depth.REPOSITORY. Multiple sequence alignments.rar: All 10 alignments used to build phylogenetic trees.REPOSITORY. Wolbachia multi-locus backbone tree.pdf: Full multi-locus, backbone Wolbachia phylogenetic tree.REPOSITORY. Serratia 6-locus and auto-MLST trees.pdf: Full 6-locus and auto-MLST Serratia phylogenetic tree.REPOSITORY. iNaturalist Pn. corruscus observations - 01-24-2025.xlsx: All observations of Photinus corruscus reported on iNaturalist, including tagged observations showing mating specimens in relevant states and years to our study.REPOSITORY. Specimen pictures.rar: Photos of insect specimens.