Barramundi Liver Raw Sequence Reads

Total RNA extracted from liver from 12 individuals and pooled in equal quantities. Individual RNA was from BAR 10-3 timecourse post feeding experiment. Liver RNA extracted from different times after feeding.\nPooled animals: 2 animals (T0-J and K) - not fed; 3 animals; 1hr (T60-I,J,K) and 2hrs (T120-I,J,K) after feeding (6 total); 1 animal - 4hrs (T4-I), 8hrs (T8-I), 12hrs (T12-I) and 24hrs (T24-I) after feeding\nGenerated thousands of unique barramundi sequences for gene discovery in fastq format. Used for de novo transcriptome assembly for barramundi liver.\nLab Notebook: LN2010/1717 p85\nLineage: Sample Name: 2011_04_25_NickWade_Fish_Liver\nApplication: Transcirptome Sequencing \nSample Buffer: water \n260/280 ratio: 2.0\nConc (ng/ul): 500\nVolume (ul): 480\nTotal Amount: 240ug\nSample Type: Total RNA \nLibrary Type: Shotgun library \nPooling: equal amounts (20ug) from 12 individuals \nMachine: GS-FLX Titanium\nRun type: Normal \nRun Scale: 1/4 region \nBarcode sorting: no\n\nRead count: 109,883\nTotal bases: 38,088,470\nAv Read length: 300bp\n\nFile Format: FASTQ (.fa) contains nucleotide sequence and corresponding quality scores