Transcriptome quantification using EXB molecular barcodes in bulk and single cell samples

The study goals are to utilize error-correcting molecular barcodes to accurately quantify transcriptomes. Conventional RNA-Seq studies with small input amounts suffer from amplification artifacts that impact downstream analyses. Random nucleotide barcodes have been used as a marker for unique molecules, but errors during PCR and sequencing negatively impact the quantification accuracy. We use error-correcting molecular barcodes to mitigate these errors and apply them to bulk mRNA and single cell transcriptomes.