Populus tremula x alba 717-1B4 Laccaria bicolor S238N ectomycorrhiza

Illumina technology was used to generate mRNA profiles of Populus tremula x alba 717-1B4 control roots and Laccaria bicolor S238N ectomycorrhiza. Total RNA was extracted, TruSeq mRNA Stranded libraries were constructed and and sequenced (2 x 150 bp Illumina HiSeq3000) at the Genotoul sequencing facilities (Toulouse, France). Raw reads were trimmed for low quality (quality score 0.05), Illumina adapters and sequences shorter than 15 nucleotides and aligned to the Populus trichocarpa v4.1 primary transcripts available at Phytozome (https://phytozome-next.jgi.doe.gov/info/Ptrichocarpa_v4_1l) using CLC Genomics Workbench v24. mRNA profiles of control roots and Laccaria bicolor S238N-Populus tremula x alba 717-1B4 in vitro ectomycorrhiza after 14 days of contact were generated by Illumina HiSeq 3000 (2x150bp).