Human iPSC-derived microglia assume a primary microglia-like state after transplantation into the neonatal mouse brain [Single Cell RNAseq]

iPSC derived microglial precursors were tranplanted into the brains of neonatal mice, FACS sorted out based on GFP+ following 2 months, and compared to in vitro differentiated iPSC derived microglia from the same precursors. Overall design: Microglial Precursors were either transplanted into neonatal mice or plated for in vitro differentiation. Transplanted GFP+ cells and in vitro differentiated cells were collected for FACS sorting and scRNA library prep following 60 days. In vivo samples from four chimeric mice (n=4) and two in vitro samples (n=2) were collected. For mice, n=one mouse. For in vitro differentiated microglia, n=3 pooled well of a 6-well plate.