Genome assembly and whole-genome resequencing study of Butuo Black sheep（Ovis aries）

Butuo Black Sheep (BBS) exhibits excellent high-altitude adaptability, physical condition, and meat production performance. Despite advancements in genomic research, the genome of BBS remains poorly understood. In this study, we generated a high-quality chromosome-level genome with a size of 2.95 Gb, a contig N50 of 71.45 Mb, and a scaffold N50 of 92.26 Mb. Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis indicated a genome assembly completeness of 95.9%. De novo genome prediction showed that repetitive sequences accounted for 47.74% of the entire genome, with Long Interspersed Nuclear Elements (LINEs) comprising the largest proportion.<br>Additionally, we report 50 sheep genomes generated by sequencing DNA samples on the Illumina NovaSeq 6000 platform, with an average depth of 10.36X and paired-end read lengths of 150 bp. A total of ~1.2 TB of raw data was produced, and 99.9% of clean reads successfully mapped to the sheep reference genome assembly with a coverage of 99.6%. This BBS genome dataset provides a valuable resource for studying the genetic diversity and evolutionary patterns of BBS.