The mechanisms by which RhoA activity and associated synaptic effects are controlled by the DISC1 scaffolding-like protein

This dataset contains the raw data for the manuscript entitled \"The mechanisms by which RhoA activity and associated synaptic effects are controlled by the DISC1 scaffolding-like protein.\" The raw data corresponding to each main figure and supplementary figure of the article are shown in a spreadsheet. The spreadsheet tabs clearly indicate which figure of the paper the dataset corresponds to. Also, the microscopy dendritic spine images and dendrite tree images used for quantification are compiled and shared. The raw microscopy images are from primary neuronal cultures transfected with full-length PDZ-RhoGEF (i.e, wild-type PDZ-RhoGEF) or PDZ-RhoGEF containing deletions of the enzymatic domain of the PDZ domain. The quantification of the microscopy images demonstrates the effects of these PDZ-RhoGEF constructs on dendritic spine density, dendritic spine morphology, and the length and branching of the apical and basal dendrite trees.
, Primary Neuronal Transfection:
Primary cortical rat neurons were transfected with cDNA between DIV21-DIV23 with Lipofectamine 2000. cDNA of Myc-tagged PDZ-RhoGEF constructs in combination with GFP were mixed with Lipofectamine in DMEM containing 10 % fetal bovine serum and incubated at 37 °C for 30 minutes, then 100 µl of this combination was added to the cells. 4 hours later, the cell media was removed, and 500 µl of old neurobasal media that was not exposed to Lipofectamine (removed just prior to the addition of the cDNA+Lipofectamine) and 500 µl of fresh neurobasal media supplemented with B27 (Gibco), DL-AP5 (Tocris), penicillin/streptomycin, and L-glutamine was added to each culture dish.
Cell culture fixation and immunocytochemistry for dendritic spine and Sholl analysis:
 Five days following transfection with cDNA, primary cortical neurons were fixed (between DIV26-DIV28) by the application of a combination of 3.7 % formaldehyde and 4 % sucrose for 10 minutes at room temperat..., # Data from: The mechanisms by which RhoA activity and associated synaptic effects are controlled by the DISC1 scaffolding-like protein

Dataset DOI: [10.5061/dryad.jwstqjqq2](https://doi.org/10.5061/dryad.jwstqjqq2)

## Description of the data and file structure

The dataset spreadsheet is the raw data in an Excel file for the above-named manuscript. Each tab detailed below corresponds to the raw data for the indicated figure number in the above-named manuscript. For each tab, a brief overview of the experiment, the abbreviation used, and the processing of the raw data is described.

The interaction between the DISC1 protein and active RhoA and PDZ-RhoGEF was examined using overexpression in HEK293T cells. The effects of PDZ-RhoGEF deletion constructs on active RhoA levels were also assessed, as were the effects of DISC expression on the membrane vs. cytosolic localization of PDZ-RhoGEF in cells using a fractionation procedure. Finally, the effects of PDZ-RhoGEF deletion constructs on ...,