Genome-wide binding of Cmi in Drosophila. Genome-wide binding of Cmi in Drosophila

Cmi (also known as Lpt) is the PHD finger domain-containing subunit of the Drosophila MLR COMPASS (Cmi-Trr) complex. ChIP-seq analysis of Cmi at different stages of Drosophila development suggests dynamic genome localization of the Cmi/MLR complex on enhancers and promoters during Drosophila development. Overall design: We used an antibody against Cmi/Lpt (raised against amino acids residues 647-1069) to monitor genome-wide binding of this subunit of the Drosophila MLR complex. ChIPs were performed using either S2 cells or whole animals at larval and prepual stages. Previously published embryo Cmi data were analyzed in parallel.