Ancient sedimentary plant DNA and pollen dataset from Bolshoe Toko Lake, southeastern Siberia

Here we provide a dataset on genetic and pollen plant diversity retrieved
from sedimentary ancient DNA (sedaDNA) of the Bolshoe Toko lake from
southeastern Siberia. Our dataset encompasses sedaDNA sequence data of 54
lake sediment samples and pollen grain counts of 67 lake sediments
samples. We used a PCR-based metabarcoding approach combined with
Next-Generation Sequencing to assess the past, local plant diversity
around the analysed lake localities. As a plant specific metabarcode we
applied the established chloroplastidal P6 loop trnL marker for plant
diversity assessment. PCR products were sequenced on one Illumina
sequencing run (HUA-9). Pollen and non-pollen palynomorphs (NPP) were
identified using a light microscope using pollen atlases and pollen
reference collections at the Arctic and Antarctic Research Institute
(Sankt-Petersburg) and the Alfred Wegener Institute.