ATAC-Seq experiment of major tissues from wild-type mice under fasting and fed treatments

Male C57BL/6 mice, sourced from the Jackson Laboratory, were acquired at 12 weeks of age. The mice were housed in groups of three per cage, with unrestricted access to water and a standard chow diet. For the experimental treatments involving fasting and fed states (n = 3 per group), the mice were either provided continuous access to food or subjected to a 24-hour fasting period before euthanasia at 10 am for tissue collection. The tissues harvested included muscle, liver, heart, lungs, both white and brown adipose tissues, spleen, and kidney. These tissues were immediately preserved in liquid nitrogen post-sacrifice to maintain their integrity for subsequent analyses. Overall design: The nuclei were isolated tissues from pooled frozen tissues and sent for ATAC-Seq library preparation.The ATAC-Seq DNA library generations were processed using Kaestner lab's pipeline.The purified DNAs were amplified using TruSeq i7 index primers (Illumina) via PCR. The library quality and quantity were evaluated using Bioanalyzer and Qubit, and the final library was sequenced at NHLBI DNA Sequencing and Genomics Core.