CUT&Tag profiling of H3K27ac reveals super-enhancer reprogramming in human lung fibroblasts

Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal lung disease characterized by persistent fibroblast activation and excessive extracellular matrix deposition. Emerging evidence suggests that epigenetic reprogramming plays a critical role in fibroblast activation during fibrotic progression. In this study, CUT&Tag sequencing targeting H3K27 acetylation (H3K27ac) was performed in human lung fibroblasts (MRC-5) to profile active regulatory elements and identify enhancer and super-enhancer landscapes associated with fibrotic signaling. The dataset provides genome-wide maps of H3K27ac enrichment and supports integrative analyses linking chromatin acetylation, transcriptional regulation, and fibroblast activation.