Gene expression profile at single cell level of four PBS injected mice, six tumor-bearing mice and four tumor-bearing mice treated with anti-IL-1a.

We used single cell RNA sequencing (scRNA-seq) to analyze the diversity transcriptomic profiles micro-surgically dissected metastatic regions of the sentinel lymphonodes (3 different condition) Overall design: we micro-surgically dissected the metastatic regions of the sLNs and analyzed them by single-cell RNA sequencing Treatment groups: neg control: PBS control: tumor-bearing mice treatment: tumor-bearing mice treated with anti-IL-1a