RNA-seq analysis of Arabidopsis root in response to temperature

We reported the temperature responses in roots affect the gene expression. To identify the transcriptome profiles response to temperature, we performed an mRNA-seq experiments in Col-0, hy5-215 and chl1.5 with 2 different temperature conditions (21 and 28C). The differential expressed genes in response to temperature were analyzed. It has 36 samples in total, with 3 biogical replicates for each condition. 4 days old seedling of Col-0, hy5-215, and chl1.5 were grown on 1/2 ms plate and transfer to the either 21 or 28C for additional 5 days, respectively.