Gene expression data from human omental adipose tissue

Among 226 morbidly obese patients who underwent gastric bypass surgery between 2013 and 2014 as part of the A Biological Atlas of Severe Obesity (ABOS) study (ClinicalTrials.gov; NCT01129297), 18 women who gave informed consent were recruited in this study for immunophenotyping and microarray analyses of omental adipose tissue (AT). We characterized T and NK cell populations in omental AT from morbidly obese women with varying levels of IR. AT IFN-gamma NK cells, but not CD4, CD8 or gamma delta T cells, were positively correlated with glucose levels, glycated hemoglobin (HbA1c), and IR. AT NK cells were linked to a pro-inflammatory gene expression profile in AT and developed an effector phenotype in response to IL-12 and IL-15. Moreover, integrated transcriptomic analysis revealed a potential implication of AT IFN-gamma NK cells in controlling adipose tissue inflammation, remodeling, and lipid metabolism, suggesting that NK cells are involved in metabolic homeostasis in visceral AT in humans. 18 morbidly obese women were recruited in this study for immunophenotyping and microarray analyses of omental AT. Omental AT samples were used for RNA isolation and flow cytometry. Glucose, insulin, glycated hemoglobin (HbA1c), and C-peptide were measured after overnight fasting and after a 75-g oral glucose tolerance test.